University of Rajasthan Paper - M.Sc. Biotechnology

Important Note:

1. Question 1 is compulsory and to be answer at one place in the answer book

2. Attempt five question out of nine.

Animal cell science and Technology

2012

1.A. Define the following:

(a) Primary culture

(b) Simple growth medium

(c) Cell cloning

(d) Histolytic culture

(e) Somatic culture

B. Write the full form of:

(a) ES

(b) RCCS

(c) HEPA

(d) FBS

(e) CHO

C. Fill in the blanks:

(a) Serum contains..........which promotes cell proliferation.

(b) Freshly isolated cell cultures are called........................

(c) The process of ovary culture is known as ..................

(d) ..............are pluripotent cultured cells derived from early pre-implantation embryos.

(e) During cryopreservation, the temperature of liquid nitrogen remains at....................

D. Write True or False:-

(a) Temperature and pressure of an autoclave is maintained at 121c and 15 lb/in2.

(b) Precursor cells are not derived from stem cells.

(c) A vaccine is a preparation containing a pathogen either in attenuated or inactivated state.

(d) Protein free media contain protein constituent necessary for culture of the cells.

(e) Plasma clot is a natural media.

2. Explain the process of animal tissue culture and the equipment required.

3. Describe the basic cell technique to culture a mammalian cell in vitro. Also illustrate the process of cell separation and maintenance of cell culture.

4. What is meant by cell cloning? Explain types of cloning and micromanipulation.

5. Write an account of stem cell culture and embryo stem cell culture with their application

6. Write an essay on "Programmed cell death"?

7. What do you mean by primary cell culture? state various stages of primary cell culture.

8. Define a balance salt solution and explain the role of carbon dioxide, serum, and supplements in it?

9. Briefly describe:

(a) Measurement of cell death

(b) Genomic library

(c) Cell transformation

(d) Cell syncronization

Plant Biotechnology

1.A. Fill in the blank's
(a) The variations observed during tissue culture of some plants are known as...........
(b) A technique of using small metal particles coated with desired genes in gene transfer is called.........
(c) DNA fingerprinting is based on.........
(d) The techniques used to generate 'golden rice' is known as........
(e) During media preparation the thermolabile chemicals are sterilized by..........
(f) The flaver savr tomato was produced by.........
(g) The beta-gal reporter gene is encoaded by.........
(h) The CaMV 35S promoter.........

B. Define the following ?
(i) Cybrid
(ii)  Aseptic culture

(iii) Bt gene
(iv) Electroporation
(v) DNA vaccine
(vi) Dimethyl sulfoxide
(vii) PR protein
(viii) ACC Synthase
(ix) Glycophosphate
(x) Cellulase
(xi) Interferon
(xii) How does the bt gene kill insects?

2. Describe the various applications of plant tissue culture techniques.

3. What do you mean by somatic hybridization? Discuss the techniques and achievements of somatic hybrids.

4. What are binary vectors? Discuss the mechanism of T-DNA transformation.

5. What do you mean by promoter clearance? Discuss various types of eukaryotic promoters.

6. Discuss briefly all the tools and techniques used in r-DNA technology

7. What do you mean by post-harvest losses? Discuss that biotechnology and genetic engineering can prevent post-harvest losses?

8.  Discuss the principles and methodologies of metabolic engineering.

9. Discuss the advantages and disadvantages of various genetic marker's.

Bio-process Engineering and Technology

1. Answer briefly:-
(a) Shear rate in bioreactors
(b) Cell disruption
(c) Fermented food
(d) Antifoams
(e) Enrichment cultures
(f) Quality control of preserved stock cultures
(g) Air sterilization
(h) Gluconic acid
(i) Fed-batch culture
(j) Single cell protein

2. Write short note on:
(i) Bioreactors types and classifications
(ii) Media sterilization

3. Differentiate between:
(i) Batch and continuous bioreactors
(ii) Sterilization and pasteurlization
(iii) Foam separation and precipitation
(iv) Drying and crystilization

4. Describe various steps in the industrial production of citric acid.

5. Explain the process of whole cell immobilization and mention its industrial applications

6. write short notes on:-
(i) Configuration and application of photobioreactors
(ii) Food packing

7. Give an account of the various steps involved in downstream processing.

8. write a short notes on:-
(i) Canning
(ii) Technology for cheese production

9. Describe in detail the use of microbes in minerals beneficiation

Bio-resources and Environmental Biotechnology

1.I. Fill in the blanks:

a)Name the common toxic biocide…………..

b)Indian Agriculture research Institute is situated at……….

c)The concept of center of origin of crop plants given by………

d)Name a GN food crops………

e)An Indian Patent is valid for a period of……..

f)Any product, by-product or residue that can not be used profitably is called…..

g)The compounds that resist biodegradation and thereby persist in the environment are called….

h)Many xenobiotic compounds are ………in nature.

i)Rotating discs are used in biological treatment of………

j)Euphorbia Lathyris produce hydrocarbons which can be converted in to …….

      II. Write True/ False for the following:-

a)CGIR is a world famous germplasm bank.

b)Breeder’s seeds are certified seeds.

c)Plastics are biodegradable.

d)The solid wastes in water are called sludge.

e)A Patent is a right granted to an inventor by the government.

III. Write short answers:

a)What are Oxidation ponds?

b)Write names of two pesticides.

c)Write full form of CGIAR.

d)What are GM food crops?

e)What do you mean by biosafety measure?

2. What is biodiversity? Write about methods of conservation of biodiversity.

3. Write notes on a) Aerobic filters b) Bioremediation of contaminated soils and waste land.

4. Discuss some environmental problems of global significance along with solutions.

5. Write notes on a) Bioresources b) Sources of water pollution.

6. Give an account of some institution for their contribution in agriculture research and development.

7. What are molecular markers? Give their types and applications in plant breeding.

8. Describe common methods of classical plant breeding.

9. Write notes on a) Seed Biology b) Seed Banks.